pgl3basicvector质粒图谱及其指导.pdfVIP

T e c h n i c a l M a n u a l pGL3 Luciferase Reporter Vectors INSTRUCTIONS FOR USE OF PRODUCTS E1741, E1751, E1761 AND E1771. PRINTED IN USA. Revised 12/08 Part# TM033 A F 9 T M0 3 3 1 2 0 8 T M0 3 3 pGL3 Luciferase Reporter Vectors All technical literature is available on the Internet at: /tbs/ Please visit the web site to verify that you are using the most current version of this Technical Bulletin. Please contact Promega Technical Services if you have questions on use of this system. E-mail: techserv@ 1. Description 2 2. Product Components and Storage Conditions 2 3. pGL3 Vector Maps and Sequence Reference Points 2 A. pGL3-Basic Vector3 B. pGL3-Enhancer Vector 4 C. pGL3-Promoter Vector 5 D. pGL3-Control Vector 6 4. Cloning Methods 7 A. Cloning Strategies7 B. Preparation of pGL3 Vectors and Insert DNA for Cloning8 C. Transformation Protocols for pGL3 Vectors 8 D. Isolation of Plasmid DNA8 5. Transfection of Mammalian Cells 9 6. Assay of Luciferase Activity 9 7. Sequencing of Luciferase Reporter Vectors 11 8. Appendix 12 A. Common Structural Elements of the pGL3 Luciferase Reporter Vectors 12 B. Advantages of the pGL3 Vectors 13 C. The pGL3 Vectors luc+ Gene 14 D. Mapping Genetic Elements Located Within DNA Fragments16 E. Composition of Buffers and Solutions 16 F. References17 G. pGL3-Basic Vector Restriction Sites18 H. pGL3-Enha