小鼠早期胚胎发育.ppt

Cell lineages in early mouse embryo Embryo culture system for time-lapse movie acquisition of node morphogenesis Dev Cell 13, 884-896, 2007 in vitro embryo culture GFP knock-in tracing Time-lapse images of node morphogenesis Noto- Dev Cell 13, 884-896, 2007 The displacement model of endoderm morphogenesis in the mouse gastrula The dispersal model of endoderm morphogenesis in the mouse gastrula Visceral endoderm-derived cells are incorporated into the gut tube 12–18 somite embryos revealed the presence of Afp-GFP+ cells in the gut tube This was not new expression of the transgene, as GFP mRNA could only be detected in the yolk sac at this stage These visceral endoderm derived cells continue to divide and are eventually incorporated into the epithelium of the gut tube Dev Cell 15, 509-520, 2008 Cell lineages in early mouse embryo Definitive endoderm Mesoderm Ectoderm Mesendoderm CNS Epidermis Epiblast Cell lineage in early mouse embryo E7.0 T Ectoderm Mesendoderm Bra Wnt3 E7.5 Mesendoderm markers Gene expression and regionalized allocation of tissue progenitors Axial mesoderm Definitive endoderm Paraxial mesoderm Lateral plate mesoderm Extraembryonic mesoderm Neuroectoderm ? The markers of germ layers Definitive endoderm Mesoderm Ectoderm Mesendoderm Neural Epidermis Nkx2.5 Sox17 Nodal, T, Wnt3, Gsc Epiblast Mesp1 Mixl1 Flk1 Gata4 Gata6 Foxa2 Sox1 Pax6 Nestin Six3 Ck5 Ck8 Ck18 Zo-1 FGF5, Nodal Fate-mapping of the distal cap epiblast by carbocyanine dye labeling E6.5 Dye injection The distal cap epiblast cells migrate to the anterior neuroectoderm Development, 107, 55-67 (1989) Current Biology 1996, Vol 6 No 2 Fate maps for the ectodermal layer Wheat germ agglutinin (WGA) Development 139, 423-436 (2012) Single cell fate map of ectodermal cells in early mouse embryo E7.0, single cell injection of HRP and LRDX, and in vitro culture 24 hr Development 139, 423-436 (2012) Tissue contributions of descendant cells according to the original position of HRP-injected pr