电解质浓度改变对心磷脂囊管结构稳定性的影响Effectof.pdf

2009 年第67 卷 化 学 学 报 Vol. 67, 2009 第12 期, 1375～1382 ACTA CHIMICA SINICA No. 12, 1375～1382 ·研究论文· 电解质浓度改变对心磷脂囊管结构稳定性的影响 陈 燕a,b 李 莉*,a,b 邱 枫b 杨玉良b (a 复旦大学分析测试中心 上海 200433) (b 复旦大学高分子系 聚合物分子工程教育部重点实验室 上海 200433) 摘要 心磷脂是构成线粒体内膜的特殊磷脂组分, 是线粒体内膜不同功能态下的拓扑结构变化的主要参与者. 本实验 通过溶剂自然蒸发与微操注射两种途径, 原位研究了溶液中电解质浓度改变程度对心磷脂构成的囊管结构稳定性的影 响, 以期对线粒体功能状态的变化机理有进一步的认识. 实验发现, 即使没有蛋白质对膜曲率的调制作用, 单纯由溶 液浓度改变造成的刺激也可以引起心磷脂膜组装形态的改变. 皱缩的发生与膜表面的电势变化有关, 是可受浓度调控 的可逆过程. 关键词 心磷脂; 囊管; 结构稳定性; 组装形态 Effect of Electrolyte Concentration on the Stability of Myelin Figure Composed of Cardiolipin Chen, Yana,b Li, Li*,a,b Qiu, Fengb Yang, Yuliangb (a The Center of Analysis and Measurement , Fudan University, Shanghai 200433) (b Key Laboratory of Molecular Engineering of Polymers of Ministry of Education, Department of Macromolecular Science , Fudan University, Shanghai 200433) Abstract In order to understand the mechanism of the transition between different mitochondrial func- tional states, an in-situ study of the stability of myelin figure composed of cardiolipin was performed by changing the electrolyte concentration of systems. The methods of changing electrolyte concentration are the solvent-evaporation method and the micro-injection method. It was found that, even without protein regula- tion, the morpholog