分子生物学实验Experimentsofmolecularbiology说课材料.pptVIP

分子生物学实验Experiments of molecular biology ;分子生物学实验室要求;实验一核酸的提取（质粒DNA的提取和纯化）;【实验原理】; 【实验步骤】 1. Pick single colony and inoculate 5 ml of LB broth containing 200 g/l Ampicillin or 1mg/5ml with a loosened cap and a piece of tape to hold it in place. Shake at 250 RPM 37oC overnight. 2. Centrifuge 1.5mL cells in 1.5 mL Eppendorf tube at top speed for 1 minute. Discard supernatant.? 3. Resuspend cell pellet in 100 ul of GTE buffer (50 mM Glucose, 25 mM Tris-Cl, 10 mM EDTA, pH 8).? Vortex gently if necessary. 4. Add 100 ul of NaOH and SDS lysis solution (0.2 M NaOH, 1% SDS) respectively. Invert t