分子标记技术20091ppt课件教学教材.pptVIP

分子标记技术;分子标记技术课程安排;;一、分子标记技术概述;形态标记;细胞学标记;生物化学标记; 分子标记; 分子标记（molecular marker）技术产生的原因：（形态性状的局限） 基于形态的遗传标记（形态标记）发展到同工酶又到DNA分子标记 DNA分子标记在分子生物学的发展过程中诞生和发展，它能够直接反映基因组DNA间的差异;First Generation: 1980s -Based on DNA-DNA hybridizations, such as RFLP. Second Generation: 1990s -Based on PCR: Using random primers: RAPD, DAF, ISSR. Using specific primers: SSR, SCAR, STS -Based on PCR and restriction cutting: AFLP, CAPs. Third Generation: recently -Based on DNA point mutations (SNP), can be detected by SSCP, DNA chip, sequencing etc. 酶切扩增多态