计算机在生物学中的作用期末试题.docxVIP

揣惴也 賞 99寸LgaoozDin潞 ￥ go齋6000 sasiH舁册专M 逐淋外范娠iH逐外 鲁东大学生命科学学学院20 11—20 12学年第1学期 《计算机技术在生物学中的应用》课程论文 课程号：2512290-200 论文题目: 1、请以E.coli heat-labile enterotoxin B subunit基因在转基因植物中高效 表达为研究题材，查询相关基因序列，相关中外文文献、结合已学知识，以其 中1篇中文文章的部分内容为素材，运用Endnote,以planta期刊为模板，书 写论文。 2、鼓励个性发展，也可不局限上述研究题材。 论文要求：（对论文题目、内容、行文、字数等作出判分规定。） 1、 基因序列查询（5分），中外文文献查询（10分）。 2、 论文目录自动生成（10分），参考文献中外文（10分），两图片合理放 在一起（15分）、论文引用（10分）、论文结构合理（15分）、正确运用多 种生物软件（20分）。 3、 统一上交以本课程论文模板为标准的论文。打印的论文除上交一份纸质 论文外,还需以学号+姓名为标题向qiushen弊han￡001@126?com发电子版。 （5分） 教师评语: 教师签字： 年 月 日 正文 Oral Vaccination of Baculovirus-Expressed VP2 DisplaysEnhanced Protection against White Spot Syndrome Virusin Penaeus monodon Syed Musthaq S1, Jimmy Kwang 1,2* An imal Health Biotech no logy, Temasek Lifesciences Laboratory, National University of Singapore, Singapore, Singapore, 2 Department of Microbiology, Faculty ofMedicine, National University of Singapore, Singapore, Singapore Abstract White Spot Syn drome Virus (WSSV) is an in fectious pathoge n of shrimp and other Crustacea ns, and neither effective vaccines nor adequate treatme nts are curre ntly available ? WSSV is an en veloped dsDNA virus, and one of its major envelopeproteins, VP28, plays a pivotal role in WSSV infection. In an attempt to develop a vaccine against WSSV, we inserted theVP28 gene into a baculovirus vector tailored toexpress VP28 on the baculovirus surface under the WSSV ie1 promoter (Bac-VP28). The Bac-VP28 incorporated abundant quantity (65.3 mg/ml) of VP28? Shrimp were treated by oral and immersion vaccination with either Bac-VP28 or wild-type baculovirus (Bac-wt). The treatment was followed by challenge with WSSVafter 3 and 15 days? Bac-VP28 vaccinated shrimp showed significantly higher survival rates (oral: 81.7% and 76.7%;immersion: 75% and 68.4%) than Bac-wt or non-treated shrimp (100% mortality). To verify the protective effects of Bac-VP28, we examined in vivo expression of VP28 by immunohistochemistry and qua ntified the WSSV copy nu mber by qPCR .In additi on