大鼠移植心脏的细胞凋亡及其与急性排斥反应的关系.docVIP

???? 大鼠移植心脏的细胞凋亡及其与?急性排斥反应的关系 ???? ????【摘要】　目的　观察移植心脏的细胞凋亡现象及其与急性排斥反应的关系。方法　建立大鼠异位心脏移植模型，用HE染色和原位末端标记(TUNEL)技术检测移植心脏切片，进行排斥反应的病理分级，计算凋亡指数(AI)。 结果　发生凋亡的细胞主要是心肌细胞，在各级排斥反应中均可见凋亡细胞存在，且AI与急性排斥反应的分级成正相关；各级的AI与0级（无排斥反应）比较，差异均有显著性。结论　细胞凋亡与移植心脏急性排斥反应的严重程度显著相关，凋亡指数可作为判断移植物损伤程度的指标，对急性排斥反应的诊断有一定参考价值。　　【关键词】　脱噬作用；心脏移植；移植物排斥；原位缺口末端标记 Apoptosis in cardiac allograft and its relation with acute rejection in rats ZHONG Hongxing, HAN Hui, ZHANG Yongshang.（Department of Urology, The First Affiliated Hospital of Jinan University Medical College, Guangzhou 510632, China） 　　【Abstract】　Objectives　To determine whether apoptotic cell death is involved in rat cardiac allograft rejection and investigate the relevance of apoptosis with acute rejection and its implication. Methods　Groups of Wistar rats underwent heterotopic heart transplantation from allogeneic SD or syngeneic Wistar rats. The cardiac grafts were harvested at 1, 3, 5, or 7 days after transplantation and underwent the detection of apoptotic cell death using in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL). Histopathological rejection grade and apoptotic index (AI) were analyzed. Results　The incidence of apoptotic cells was increased steadily over time in allografts, in contrast to syngeneic grafts. The apoptotic cells in allografts were mainly cardiac myocytes and few infiltrating lymphocytes. The AI of rejection grade 1, 2, 3 and 4 was significantly higher than that of rejection grade 0 (P0.01). Conclusions　TUNEL can display apoptosis of single cell in situ. Apoptosis is an important mechanism of tissue injury in acute cardiac allograft rejection in rats. Myocyte apoptosis can be used as a valuable index to estimate the injury of grafts and monitor acute rejection.　　【Key words】　Apoptosis；Heart transplantation；Graft rejection；In situ nick-end labeling 　　细胞凋亡是近20年来生命科学领域中最重要的进展之一，细胞凋亡的研究具有普遍的生物学意义，也是器官移植领域的一个重要课题［1，2］。我们通过大鼠心脏移植模型，用原位末端标记（TUNEL）技术［3］检测排斥反应时的细胞凋亡情况，现报告