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Chapter Eleven  Spectrophotometry Spectrophotometry, is one of the most widely used methods of analysis. It is used to measure the absorption capabilities of certain compounds with reference to wavelengths of light on the ultraviolet and visible light ranges. e.g. There is 10-8g Fe in 10ml solution, if titrate it using KMnO4 standard solution (c=2.0×10-4 mol/L), only consumed 0.02ml KMnO4 standard solution The instrumentation is readily available and generally fairly easy to operate. It is very widely used in clinical chemistry, hygiene analysi