光学显微镜应用：暗场照明.docVIP

Optical Microscopy Application: Darkfield Illumination Darkfield illumination is a technique in optical? microscopy?that eliminates scattered light from the sample image. This yields an image with a dark background around the specimen, and is essentially the complete opposite of the brightfield illumination technique. The primary imaging goal of the darkfield illumination technique is to enhance the contrast of an unstained sample, which is incredibly powerful, yet simple, for live cellular analysis or samples that have not gone through the staining process. IMAGE APPEARANCE A typical darkfie