内分泌因素影响牙槽骨修复的动物实验研究.doc

内分泌因素影响牙槽骨修复的动物实验研究 ???? 内分泌因素影响牙槽骨修复的动物实验研究 ???? 华西口腔医学杂志 2000年第1期第18卷 基础研究 ???? 作者：李德懿　刘瑷如　汪铮　胡庆沈　叶军 ???? 单位：李德懿（上海第二医科大学附属第九人民医院　200011）；刘瑷如（上海第二医科大学附属第九人民医院　200011）；叶军（上海第二医科大学附属第九人民医院　200011）；汪铮（上海第二医科大学图像分析室）；胡庆沈（上海第二医科大学图像分析室） ???? 关键词：内分泌因素；牙槽骨修复；碱性磷酸酶；酸性磷酸酶；图像分析 ???? 　　摘　要：目的:明确内分泌因素对牙槽骨修复过程中碱性磷酸酶(ALP)和酸性磷酸酶(ACP)活性的影响,并探讨牙槽骨吸收修复机制。方法:24只狗的牙槽骨二壁骨袋模型,随机分成4组,采用组织学检查、酶组织化学和全自动图像分析等技术定量分析去除卵巢、诱发实验性糖尿病及提高甲状腺素水平情况下,骨缺损修复过程中ALP和ACP活性变化。结果:甲状腺素组除6个月组ACP活性较高外,ALP与ACP活性均较对照组低,有统计学差异(P＜0.01);糖尿病组和卵巢切除组仅ALP降低有统计学差异(P＜0.05或P＜0.01),酶的活性与骨形态结构变化相吻合。结论:牙槽骨修复受全身内分泌因素的调控,且与其破骨细胞和成骨细胞的酶活性密切相关。 ???? Animal Experimental Study on Endocrine Factors Affecting Alveolar Repair ???? Li Deyi　Liu Airu　Ye Jun ???? 　　（The Ninth Affiliated Renmin Hospital, Shanghai Second Medical University） ???? 　　Wang Zheng　Hu Qingshen ???? 　　（Image Analysis Office, Shanghai Second Medical University） ???? 　　Abstract：Objective：To investigate the endocrine effects on activities of alkaline phosphatase (ALP) and acid phosphatase (ACP) and to obtain the mechanism of the resorption and regeneration of the alveolar bone.Methods: 24 dog models with two-side osseous infrabony pocket were divided into 4 groups randomly and were studied by histologic examination, enzyme cytochemistry and image analysis system.Results: The activities of ALP and ACP, except ACP in the sixth month significantly decreased in the group of thyroidism comparing with that of the control group (P＜0.01), but only the activities of ALP significantly decreased in groups of ovariectomy and diabetes (P＜0.05, P＜0.01), and there was close relationship between enzyme activities and histopathologic variations.Conclusion: Alveolar bone repair was regulated by systemic endocrine factors and was associated with the activities of ALP and ACP osteoblasts of and osteoclasts. ???? 　　Key words：endocrine factor alveolar repair alkaline phosphatase (ALP) acid phosphatase (ACP) image analysis system▲ ???? 　　牙槽骨吸收是牙周病进展期的重要特性,目前对牙槽骨破坏修复的认识多数来自体外实