水稻光温敏核不育系的ISSR和SSR遗传分析比较_图文.doc

研究报告 RESEARCH REPOR T 水稻光温敏核不育系的ISSR 和SSR 遗传分析比较 李进波2,3　江良荣3　李春海1　牟同敏13 1华中农业大学作物遗传改良国家重点实验室, 武汉, 4300702湖北省农科院杂交水稻工程技术研究中心, 武汉, 4300703海南省农作物分子育种重点实验室, 三亚, 572025 3通讯作者, tongmin @public. wh. hb. cn 推荐人, 方宣钧 摘要 本研究应用ISSR 和SSR 技术建立了24个水稻光温敏核不育系的DNA 指纹图谱, 利用13个ISSR 引物和20对SSR 引物, 分别获得174个多态性片段和62个多态性片段, 平均每个ISSR 引物检测到13138个多态性片段, 远远高于SSR 引物的检测率。根据遗传距离进行的聚类分析表明, 利用这两种标记所得的聚类结果十分相似, 24个材料被聚为粳型, 偏籼型和籼型三个类群, 在籼型不育系类群内, 又可明显的分成三个亚类群, 其中7个安农S -1衍生的不育系聚为一类, 与农垦58S 衍生的不育系有明显的遗传差异。根据两种标记计算的遗传距离及其遗传关系, 所得的结果仍有一些差异, 但总体趋势是一致。研究结果表明, ISSR 和SSR 标记适用于构建DNA 指纹图谱, 进行分类鉴定和遗传分析。 关键词 水稻, 光温敏核不育系, ISSR , SSR , 遗传分析 Comparisons of G enetic Analysis Based on ISSR and SSR in P GMS and TGMS Rice Lines Li Jinbo 2,3　Jiang Liangrong 3　Li Chunhai 1　Mou tongmin 13 1National K ey Laboratory of Crop G enetic Improvement , Huazhong Agricultural University , Wuhan , 4300702Hybrid Rice Research and Development Center , Hubei Academy of Agricultural Sciences , Wuhan , 4300703Hainan Provincial K ey Laboratory of Crop Molecular Breeding , Sanya , 572025 3Corresponding author , tongmin @public. wh. hb. cn Communicated by Fang X. J. ABSTRACT In this study , DNA fingerprints of 24elite photoperiod 2sensitive genic male sterile (P GMS and thermo 2sensitive genic male sterile (TGMS rice lines were established using inter 2simple sequence repeats (ISSR markers and simple sequence repeats (SSR markers. 13ISSR primers could amplify 174polymorphic bands and 20SSR primers could amplify 62polymorphic bands. The numbers of average polymorphic bands produced by per ISSR primer are 13138more than those produced by per pair of SSR primer. The results showed that 分子植物育种,2003年, 第1卷, 第1期, 第42—47页 clustering analysis based on ISSR data is very similar to that based on SSR data. These 24sterile lines used in this research were divided into three groups , i ndica group , i ndica 2like and japonica group. In the i ndica group , 19sterile lines were also clustered into t hree sub groups. 7sterile lines derived f rom A nnon g S