chiral separation methods for pharmaceutical and biotechnological products (ahujachiral separation methods) chiral discrimination study for polysaccharide-based chiral stationary phases英文精品课件.pdfVIP

CHAPTER 6 Chiral Discrimination Study for Polysaccharide-Based Chiral Stationary Phases YUN K. YE Analytical Research and Development, Bristol-Myers Squibb Company, New Brunswick, New Jersey 1 INTRODUCTION Although chirality was discovered more than a century ago, chiral separation is still perceived as one of the most challenging tasks for scientists in the separa- tion field. In fact, significant progress has only been made within the last two decades, due to the intense levels of interest in the development and applica- tion of chiral separation techniques in biological research fields, especially in the pharmaceutical industries [1–5]. Chiral separation within the pharmaceutical area is essential for investigation of the pharmacokinetical, physiological, toxi- cological, and metabolic activities of both enantiomers. There is a broad range of examples concerning the means by which the stereoisomers of drugs show differences in terms of their bioavailability, distribution, metabolism, and excre- tion behavior. Examples also exist in which stereochemical parameters have a fundamental significance in their action and disposition in biological systems. Currently, evaluation of chiral purity is used routinely to monitor the stereose- lective synthesis, pharmaceutical research and development, and quality control for the pharmaceutical production of finished chiral drugs. Chiral HPLC is undoubtedly the most important technique for the separation and quantitation of enantiomers with respect to its availability, versatility, simplic- ity, and ability to scale up easily from an analytical scale to semipreparative and preparative scales [6,7]. Direct separation of enantiomers by high-performance liquid chromatography (HPLC) has become one of the most useful procedures Chiral Separation Methods for Pharmaceutical and Biote