基于转录组学和代谢组学研究梨花青苷和原花青苷合成调控机制.pdfVIP

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基于转录组学和代谢组学研究梨花青苷和原花青苷合成调控机制.pdf

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符号说明 ANS: anthocyanidin synthase, 花色素合成酶 Bp: base pair, 碱基对 cDNA: complementary DNA, 互补DNA Cef: cefotaxime, 头孢霉素 CF: clapp favorite, 茄梨 CHI: chalcone isomerase, 查尔酮异构酶 CHS: chalcone synthase, 查尔酮合成酶 ddH O: distilled and deionized water, 重蒸水 2 DEGs: differentially expressed genes, 差异表达基因 DFR: dihydroflavonol reductase; 二氢黄酮醇还原酶 DNA: deoxyribonucleic acid, 脱氧核糖核酸 F3H: flavonoid 3-hydroxylas, 黄烷酮3-羟化酶 GSTs: glutathione S-transferases, 谷胱甘肽S-转移酶 Kan: kanamycin, 卡那霉素 mRNA: messenger ribonucleic acid, 信使核糖核酸 MS: Murashige and Skoog, MS 培养基 OD: optical density, 光学密度 PAs, procyanidins, 原花青苷 PCR: polymerase chain reaction, 聚合酶链式反应 qRT-PCR: quantitative reverse transcription PCR, 荧光定量反转录PCR RCF: red clapp favorite, 红茄梨 TFs: transcription factors, 转录因子 UFGT: UDP-glucose flavonoid 3-Oglucosyl transferase, 类黄酮3,5-糖苷转移酶 WT: wild-type, 野生型目录中文摘要 ····························································································· Ⅰ 英文摘要······························································································Ⅲ 1 前言··································································································· 1 1.1 植物中的花青苷·················································································· 1 1.2 果实花青苷的生物合成········································································· 1 1.2.1 花青苷合成相关结构基因 ··································································· 2 苯丙氨酸解氨酶（PAL ）································································· 2 查尔酮合成酶（CHS ）···································································· 2 查尔酮异构酶（CHI ）····································································· 2 黄烷酮3-羟化酶（F3H ）································································· 3 二氢类黄酮还原酶（DFR ）······························································ 3 花色素合成酶（ANS ）·····················