实验四蛋白质序列结构获取和显示精析.pptVIP

基因与蛋白质组学数据分析 实验四:蛋白质序列、结构的获取和显示 杜娟 dujuannx@126.com  实验项目四:蛋白质序列、结构的获取和显示 实验目的和要求 掌握蛋白质序列数据库 Uniprot的查询方法 及格式特点 ·掌握蛋白质结构数据库PDB的及格式特点 掌握蛋白质结构显示软件 Pymol的使用  UniPro数据库 简介 UniProt Universal Protein resource 收录蛋白质序列目录最广泛、功能注释最全 面的数据库; 包含三个子库: UniProtKB(UniProt Knowledgebase) UniRef(UniProt Reference Clusters) UniParc (Uniprot Archive)  2.数据来源。 Uniprot EMBL-EBI :BR SIB European Protein Bioinformatics SIB Swiss Information Institute(EMBL Institute of Resource(pir Bioinformatics Swiss-Prot and TrEMBL Protein Sequence Database(PIR-PSD  UniProt. BLAST Abgn Retrieve/ID mapping The mission of uniProt is to pravide the scientific community with a comprehensive, high-quality and freely accessible resource of UniProtKB UniRef UniParc Proteomes ot knowledgebase 个 Swiss-Prot (549 008) Manually annotated Supporting data and reviewed Subcellular locations TrEMBL LIterature citations Taxonomy annotated and not Cross-ref. databases Keywords Getting started You Tube UniProt data UniProt的网址:htt:/ wwuniprot. org  3数据查询 Uniprot检索号,包括6个字符串,可 由大写字母A~Z和数字0~9组合而成。 也可以用关键词检索  检索演示 例1:查询草履虫细胞周期蛋白依赖的蛋白激 酶(CDK2)的结构数据 (1)登陆 Uniprot网站htp:// ww. (2)在搜索栏选中 Protein knowledgebase( UniProtKB)”,在文本框中 输入“ Paramecium tetraurelia CDK2”,单击 Site search按钮,出现结果。  UniProt i Paramecium tetraurelia CDK2 BLAST AI The mission of UniProt is to provide the scientific community with a comprehensive, high-quality and freely accessible resource of protein se UniProtKB UniRef UniParc Proteomes uniProt know ledoebase Sequence clusters Sequence archive Forthcor 果↑ Planned Swiss-Prot(549, 008) nt prot E ei Manually annotated Supporting data and reviewed. to UniPro lanes TrEMBL(50.011,027) Ltera ture citations Taxonomy Subcellular locations Automatically UniProt annotated and no Cross-ref databases 电News Getting started Tube UniProt data Protein 8  UniPro UniProtkB- paramecium tetraurelia cdk2 UniProtKB results