剖析转录因子结合位点.pptVIP

三生物倍息堂 第六章 基因预测和基因结构分析 10 自重01010自官 基因组测序策略 Hierarchical Shotgun Sequencing Method Whole Genome Shotgun Sequencing Method 之 Genome NA Gemmir DNA EAC LIbrary Cleats Canti Map CCAYSNCHGTGACCOG CACAACCHE CCATTICBRGTMCCICFARC CIVIEACACLAGISWTACISREE 華十睾大浮 http://www.bio.davidsonedu/courses/genomics/method/shotgun.html Genome sequencing: QUICKER, SMALLER, CHEAPER Table 1. Second-generation DNA sequencing technologies Sequencing by Read- References megabase Emulsion PCR s500,000 Solexa Yes subst.36 0-base encoding) Emulsion PCR Ligase(nanamer 13,2Q Single 18,30 ce with which the field is moving makes it tickly outdated. vendors including Roche Nature Biotechnology 26, 1135-1145 (2008) 13 years 1 d $3 billion 1000 華十睾大浮 http://www.ncbi.nlm.nihgov/genomes/static/gpstat.htr Genome sequencing projects statistics Draft assembly 966 Yamma 41 959121113 cutworms roundeye Amphibians Reptiles land plants 1122329 Green algae omce tes Ler Ingl Protists Kinetoplasts 3132 119122 m:1 Applications of sequencing Table 2. Applications of next-generation sequencing .Praviaustabla + Figures and tablas Refs Comprehensive polymorphism and mutabon discovery in individual human ation sequencing 46,4,484950,51r52 Discovery of inherited and acquired structural variation Metagenomic sequencing Discovery of infect and commensal fiora Quantification of gene expression and alternative splicing: transcript 65758,59,60,51,62,63 Sequencing of bisulfite-treated DNA Detemining pattems of cytosine methylation in genomic DNA 665,5 67,686869,70 uclease fragmentation and Molecular barcoding Multiplex sequencing of samples trom mulbpleindiduals identifying new genes Nature Biotechnology 26, 1135-1145 (2008) looking at chromosome organization and structure finding gene regulatory sequences 華十睾大浮 comparative genomICs Where are the Genes in the genome? A0 CTCHCTIGICHGGTTCHGMTGGIGNOGHICHTHGGTUG-T Gene a CCACATATCAAMGAAGIGTACAGGCOGNGGHCATCAATGGHGAAATTTAT GATGAGTACGYCGIGGAGYGGHTGITOCAGTGTAGTT