内切酶识别位点.docVIP

不同内切酶对识别位点以外最少保护碱基数目的要求 酶 寡核苷酸序列 切割率% 2 hr 20 hr Acc I GGTCGACC CGGTCGACCG CCGGTCGACCGG 0 0 0 0 0 0 Afl III CACATGTG CCACATGTGG CCCACATGTGGG 0 90 90 0 90 90 Asc I GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA 90 90 90 90 90 90 Ava I CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA 50 90 90 90 90 90 BamH I CGGATCCG CGGGATCCCG CGCGGATCCGCG 10 90 90 25 90 90 Bgl II CAGATCTG GAAGATCTTC GGAAGATCTTCC 0 75 25 0 90 90 BssH II GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA 0 0 50 0 0 90 BstE II GGGT(A/T)ACCC 0 10 BstX I AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT 0 25 25 0 50 90 Cla I CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG 0 0 90 50 0 0 90 50 EcoR I GGAATTCC CGGAATTCCG CCGGAATTCCGG 90 90 90 90 90 90 Hae III GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA 90 90 90 90 90 90 Hind III CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG 0 0 10 0 0 75 Kpn I GGGTACCC GGGGTACCCC CGGGGTACCCCG 0 90 90 0 90 90 Mlu I GACGCGTC CGACGCGTCG 0 25 0 50 Nco I CCCATGGG CATGCCATGGCATG 0 50 0 75 Nde I CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC 0 0 0 0 75 75 0 0 0 0 90 90 Nhe I GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG 0 10 10 0 25 50 酶 寡核苷酸序列 切割率% 2 hr 20 hr Not I TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA 0 10 10 25 25 0 10 10 90 90 Nsi I TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT 10 90 90 90 Pac I TTAATTAA GTTAATTAAC CCTTAATTAAGG 0 0 0 0 25 90 Pme I GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG 0 0 0 75 0 25 50 90 Pst I GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT 0 10 90 90 0 0 10 90 90 0 Pvu I CCGATCGG ATCGATCGAT TCGCGATCGCGA 0 10 0 0 25 10 Sac I CGAGCTCG 10 10 Sac II GCCGCGG