荧光素酶报告基因检测试剂盒说明书.pdf

T e c h n i c a l M a n u a l Dual-Luciferase? Reporter Assay System INSTRUCTIONS FOR USE OF PRODUCTS E1910 AND E1960. PRINTED IN USA. Revised 8/06 Part# TM040 AF 9 T M0 4 0 0 8 0 6 T M0 4 0 ? Dual-Luciferase Reporter Assay System All technical literature is available on the Internet at: /tbs/ Please visit the web site to verify that you are using the most current version of this Technical Manual. Please contact Promega Technical Services if you have questions on use of this system. E-mail: techserv@. I. Description 2 ? A. Dual-Luciferase Reporter Assay Chemistry3 ? B. Format of the Dual-Luciferase Reporter Assay5 C. Passive Lysis Buffer6 II. Product Components and Storage Conditions8 III. The pGL4 Luciferase Reporter Vectors9 A. Description of pGL4 Vectors9 B. Important Considerations for Co-Transfection Experiments9 IV. Instrument Considerations 10 A. Single-Sample Luminometers10 B. Multi-Sample and Plate-Reading Luminometers10 C. Scintillation Counters11 V. Preparation of Cell Lysates Using Passive Lysis Buffer12 A. Passive Lysis Buffer Preparation12 B. Passive Lysis of Cells Cultured in Multiwell Plates12 C. Active Lysis of Cells by Scraping13 ? VI. Dual-Luciferase Reporter Assay Protocol14 A. Preparation of Luciferase Assay Reagent II14