核酸化学3教学内容.pptVIP

核酸化学3;Hydrolysis of RNA under alkaline conditions. The 2′ hydroxyl acts as a nucleophile in an intramolecular displacement. The 2 ′,3 ′ -cyclic monophosphate derivative is further hydrolyzed to a mixture of 2 ′ - and 3 ′ -monophosphates. DNA, which lacks 2 hydroxyls, is stable under similar conditions.;Cleavage in polynucleotide chains: a cleavage yields 5-phosphate products, whereas b cleavage gives 3-phosphate products.;(三) 酶水解;An example of nuclease specificity: The specificity of RNA hydrolysis by bovine pancreatic RNase. This RNase cleaves b at 3-pyrimidines, yielding oligonucleotides w