TRAIL联合5-氟尿嘧啶对食管癌的作用及相关机制的探讨.doc

TRAIL联合5-氟尿嘧啶对食管癌的作用及相关机制的探讨 刘霞1 程慧敏1李彬 1 李维前1 马云松1 （ 1 徐州医学院附属第三医院病理科，徐州221003 ） 摘要 目的：探讨肿瘤坏死因子相关凋亡诱导配体TRAIL联合5-氟尿嘧啶对食管癌EC1细胞的作用及可能机制。方法：通过MTT法检测TRAIL（三个浓度5、50、200ng/ml）及联合5-Fu对食管癌细胞的增殖的作用；用流式细胞术检测细胞周期改变及凋亡率，Westernblotting检测TRAIL联合5-氟尿嘧啶对食管癌细胞周期相关基因p21和p27表达的影响；免疫细胞化学检测凋亡相关基因bax、bcl-2的表达。结果：1) TRAIL能够抑制食管癌EC1细胞的增殖并随着浓度和作用时间的提高，抑制效果增强，但效果差异不明显；TRAIL与5-氟尿嘧啶联合应用对食管癌细胞的增殖抑制作用强于两者单用;2) TRAIL与5-氟尿嘧啶联合作用后的EC1出现了G0/G1期的阻滞和早期凋亡;其机制可能分别涉及p21和p27基因表达的增加以及bax基因表达的增加。结论：TRAIL与5-氟尿嘧啶联合使用具有协同抗肿瘤作用。 关键词：TRAIL、5-氟尿嘧啶、EC1、协同抗肿瘤作用 [中国图书分类法分类号] R735.1；R730.231 [文献标志码] A Study of the killing action of TRAIL and 5 - fluorouracil for esophagus cancer cell and its related mechanism Liuxia，et.al （ Department of Pathology ,Third Affiliated Hospital, Xuzhou Medical College) 【Abstract 】 Objective: To discuss the effect of TRAIL and its combination with 5- fluorineuracils on esophagus cancer EC1 cell function and the possible mechanism. Methods: Three different concentration of TRAIL 5、 50、200 ng/ml and combined with 5- fluorouracil cell multiplication suppression rate was detected through cell strain growth inhibition curve (MTT) ; the cell cycle change and apoptosis were examined through rate flow cytometry; the effect of TRAIL on cell cycle related gene p21, p27 expressions of esophagus cancer cell was detected through Western-blotting;cell apoptosis related gene bax and bcl-2expression were detected through immunohistochemistry. Results: 1）The TRAIL could suppress esophagus cancer EC1 cell’s multiplication and proliferation; the killing action to esophagus cancer cell was stronger when combined with 5- fluorouracil than when they were used separately. 2）After using TRAIL the esophagus cancer EC1 was blocked in G0/G1 phase, and the cells expressed the early time apoptosis. Its mechanism possibly involves the increase of p21 and p27 gene expressions separately as well as the increase of bax gene expression with the bcl-2 gene expression reduction. Conclus