宏基因组学在纤维素酶研究中的应用进展.docxVIP

微生物学通报  Jun. 20, 2015, 42(6): 1089 - 1100 Microbiology China  ? 2015 by Institute of Microbiology, CAS tongbao@  DOI: 10.13344/j.microbiol.china.140652 专论与综述 宏基因组学在纤维素酶研究中的应用进展 戴利铭1 熊彩云1 黄遵锡1,2,3,4 李俊俊1,2,3,4 唐湘华1,2,3,4 杨云娟1,2,3,4 许波1,2,3,4* (1. 云南师范大学 生命科学学院 云南 昆明 650500) (2. 生物能源持续开发利用教育部工程研究中心 云南 昆明 650500) (3. 云南省生物质能与环境生物技术重点实验室 云南 昆明 650500) (4. 云南师范大学 酶工程重点实验室 云南 昆明 650500) 摘 要：纤维素酶能降解纤维素，被广泛应用于生物修复、食品加工、化工合成等领域，开发高 活力、广底物、 耐高温高碱等极端条件的新型纤维素酶具有重要意义。 宏基因组学以特定环境样 品中微生物的基因组总和为研究对象， 避开传统的微生物分离培养过程， 为基因资源的开发、 利 用提供了新技术。文中结合本课题组的研究工作，综述了利用宏基因组学获取纤维素酶的策略，同时着重介绍利用宏基因组学从动物胃肠道、土壤等环境中获取纤维素酶的研究。 关键词 ：宏基因组学，纤维素酶，高通量筛选，未培养微生物 Metagenomics in studying cellulase DAI Li-Ming 1 XIONG Cai-Yun1 HUANG Zun-Xi 1,2,3,4 LI Jun-Jun1,2,3,4 TANG Xiang-Hua 1,2,3,4 YANG Yun-Juan1,2,3,4 XU Bo1,2,3,4* (1. School of Life Science, Yunnan Normal University, Kunming, Yunnan 650500, China) (2. Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education , Kunming, Yunnan 650500, China) (3. Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Kunming, Yunnan 650500, China) (4. Key Laboratory of Enzyme Engineering, Yunnan Normal University , Kunming, Yunnan 650500, China) Abstract: Cellulases can degrade cellulose and widely applied in bioremediation, food processing, chemical synthesis and other fields. Therefore, it is really significant to develop new types of cellulases that have high activity, broad substrate spectrum, high temperature and alkali resistance, and other anti-extreme-conditions capacity. The metagenomic technology focuses on the sum of microbial genomes in environmental samples, avoiding traditional process of microbial isolation and culture, and provides a new technology for the development and utilization of genetic resources. Combined with our own findings, this paper summarizes the strategies of gaining cellulases by use of metagenomic technology, and highlights advances in studying