行业资源库 前沿技术 前沿技术-Color-Change Immunoassay for Pathogen Detection.pptxVIP

Sundaram Gunasekaran Department of Biological Systems Engineering University of Wisconsin-Madison, USA;Food Safety: No Guarantees!;The Dirty Dozen!;Food Safety is a Serious Business;Concerns for the Food Industry;Current Methods;Ideal Food Quality/Safety Indicator;Ideal Food Quality/Safety Indicator;;Biosensors;Sensor Platforms;Sensor Platforms;Biosensor Trends;Functional Nanomaterials;BioInfoBank Institute 2009;(Weiss et al., 2006);/chicago/news.aspx?id=86701;Ideal Food Quality/Safety Indicator;Ideal Food Quality/Safety Indicator;Colorimetric Biosensors;Size-Based Optical Properties of AuNPs;Color Change with Aggregation;Research Hypothesis;Switchable Bifunctional Linker (SBL);Two-Step Colorimetric Assay;;Detection of E. coli in buffer;Detection of E. coli in tap water (pH=7.53);Detection of E.coli in Lake Water (pH=8 to 9.5); 104 CFU/400μL;Salmonella Typhimurium in Milk -- 10% Dilution;Salmonella detection in tomatoes;REVC (Range Exhibiting Visual Color change) in control and sample of S. Typhimurium (103) with SBL concentrations between 14 to 52 μg/mL in PBS. ;REVC Patterns;Assay selectivity;16;Detection of S. Typhimuirum in tomato samples. The number of bacterial cells was determined by plate count and SL-based immunoassay. The mean bacteria number was 14.6 and 16.7, respectively. ;How long does it take?;Potential “10 in 10” Test Kit;Thank You!