放线菌jxj-0110对铜绿微囊藻的溶藻活性.docxVIP

江苏农业学报(Jiangsu，ofA盱Sci．)，2014，30(1)：112～118 112 http：／／www．jsn yxb．COm 张方，李汉全，杨建远，等．放线菌JXJ-0110对铜绿微囊藻的溶藻活性[J]．江苏农业学报，2014，30(1)：112一118 doi：10．3969／j．issn．1000-4440．2014．01．019 放线茵JXJ一0110对铜绿微囊藻的溶藻活性 张 方1， 李汉全2， 杨建远2， 张炳火2 (1．九江市环境保护监测站，江西九江332000；2．九江学院生命科学学院，江西九江332000) 摘要： 为明确放线菌JXJ-0110及其代谢产物对铜绿微囊藻的溶藻活性和藻细胞生长的影响，以及菌株的分类 地位，采用热乙醇法测定藻细胞叶绿素a含量，在光学显微镜下计数藻细胞数目，并利用16S rDNA的基因序列分析确 定其所在属。结果表明：放线菌JⅪ_oll0的菌丝体具有很强的溶藻活性，且呈剂量关系。发酵上清液也具有很强的 溶藻活性，藻液(1 ml 1．0x10 7个)中加入2％(体积比)的发酵上清液，3 d后叶绿素a的去除率达到88．4％。溶藻活性成分主要为胞外水溶性物质和胞内脂溶性物质。发酵上清液的溶藻活性对高温、pH值和254 nm的紫外线处理均具有较好的稳定性，90℃水浴处理发酵上清液2 h，叶绿素a的去除率超过82％；pH 6～10处理发酵上清液2 h，叶绿素a的去除率超过82％；紫外线254 nm下照射发酵上清液2 h，叶绿素a的去除率为86．4％。16S rDNA基因序列发育分析表明，菌株JXJ-0110属于链霉菌属成员，与Streptomyces shenzhervensis 1721151的序列相似性最高(99．06％)。放线菌J)(J-01 10及其代谢产物在铜绿微囊藻水华防治方面具有潜在应用价值。 关键词：放线菌；方链霉菌；铜绿微囊藻；溶藻活性 中图分类号：X524 文献标识码： A 文章编号： 1000-4440(2014)01-0112-07 Algae-lysing activity of Microcystis aeruginosa by actinomyces JXJ-0110 ZHANG Fan91，LI Han．quan2，YANG Jian．yuan2，ZHANG Bing—hu02 (1．jiujiong Environmental Protection Monitoring Station，jiujiang 332000，China；2．College of蜘Sciences jiujiang University，Jiujiang 332000，China) Abstract：The algae—lysing activities of actinomyces JXJ-01 10 and its metabolites Microcystis o,e171笞inosa were studied by measuring chlorophyll a contents using hot—ethanol extraction method，and the taxonomic status of JXJ-01 10 was defined by a— nalysis of 16S rDNA gene sequences．The mycelia of JXJ-01 10 showed strong algae—lysing activities which was dose—related，and the removal rates of chlorophyH in舱aenuginosa increased from 60．5％to 91．2％when mycelia(fresh weight)increased from 0．5g2．0g．Thefermentationsupematantofthestrainalso exhibitedstrongalgae—lysingactivities．88．4％ofchlorophyllbe·ing wiped off three days after 2％(∥y)fermentation supernatant addition，and the biomass of algae being restrained to very lOW 1evel for more than forty days．The algae—lysing components were mainly extracellular water-soluble substances and intra