ABHD2调控肺泡Ⅱ型上皮细胞凋亡 在慢性阻塞性肺疾病发生中的作用.ppt

Wild type Abhd2Gt/Gt Nfe2 Nfe2L2 SOD1 HPRT  Figure. The expression levels of oxidation and antioxidant factors Oxidation and antioxidant factors indifference Mouse Abhd2 playes an important role in emphysema The exact mechanism was unclear But the function of hABHD2 was unclear Abhd2 features ： Abhd2 deficient mice spontaneously emphysema Abhd2 expressed in airway smooth muscle and vascular smooth muscle cells. Abhd2 deletion induced vascular smooth muscle cell migration and intimal hyperplasia BT =bronchial tract Clinical manifestations of COPD ： lung: airway remodeling, emphysema, vascular remodeling: smooth muscle cell proliferation, migration lung outside: peripheral muscle atrophy and dysfunction and other symptoms We speculate: ABHD2 associated with COPD interlobular vein Protein Similarity 98.59%/425aa (from NCBI) mAbhd2 and hABHD2 Homology Abhd2 Abhd family and Abhd2 features: Alasdair J. Edgar and Julia M. Polak. Cloning and Tissue Distribution of Three Murine α/β Hydrolase Fold Protein cDNAs. Biochemical and Biophysical Research Communications 292, 617?625 (2002) Origin: Edgar and colleagues cloned α/β hydrolase family encoding a protein from emphysema model mouse lung cDNA library in 2002 : α/β hydrolase gene 1 (Abhd1), Abhd2 and Abhd3 Structural domains: Abhd1, Abhd2 and Abhd3 may play an important role in gene function with the same structural domain Study: Associations of ABHD2 Genetic variations with Risks for Chronic Obstructive Pulmonary Disease in a Chinese Han Population Extraction and comparative analysis genomic DNA of COPD patients and normal population Screening gene SNP point of ABHD2 : from shared domain of Abhd2 The study found: Rmutants (CT / CC ), upstream of the translation initiation site in the fifth ABHD2 gene intron from the sixth exon upstream 489bp, increased the risk of COPD Conclusion: ABHD2 gene polymorphism was associated with COPD risk 1 2 3 4 Exon 5 9 10 Exon 6 7 8 Abhd2 gene NCBI r