鼠尾悬挂试验在大鼠急性坏死性胰腺炎中的实验意义.docVIP

鼠尾悬挂试验在大鼠急性坏死性胰腺炎中的实验意义 【摘要】目的:探讨鼠尾悬挂试验(TST)在大鼠急性坏死性胰腺 炎中的实验意义。方法:SD大鼠被分为三组：急性坏死性胰腺炎组(A 组，n二8)、急性坏死性胰腺炎鱼油干预组(F组，n=8)和对照组(C 组，1尸8)。观察各组大鼠胰腺组织病理变化及TST静止时间，同时测 定各组5 d后血清SOD、GSH-Px活力和淀粉酶浓度。结果M组、F组 TST静止时间各时间段均显著高于C组(P lt;0.01)；自第2天起F 组的数值均小于A组，其中第4、第5天的数值差异有显著性(分别为 Plt;0・05、Plt;0.01)o病理学检查提示F组的炎性细胞浸润显著 低于 A 组(P lt;0.01)oA 组 SOD、GSH-Px 显著低于 C 组(Plt;0.01), 而F组SOD、GSH-Px显著高于A组(P 01)。TST静止时间和病 理学改变、SOD、GSH-Px活力具有相关性(P lt;0.01)o结论:TST 静止时间在一定程度上反映了急性坏死性胰腺炎的严重程度；鱼油在 改善急性坏死性胰腺炎大鼠的氧化应激指标的同时，对大鼠的TST静 止时间产生影响。 【关键词】胰腺炎急性坏死性鼠尾悬挂实验超氧化物歧化谷 胱甘肽过氧化酶 Abstract: Objective:To investigate the significance of the tai 1 suspension test in rats with acute necrotizing pancreatitis (ANP). Methods: The male Sprague-Dawley (SD) rats were divided into three groups: ANP group (group A, n=8), ANP wi th fish oi 1 treatment group (group F, n 二8) and control group (group C, n=8) . The immobility time in the tail suspension test and the grade of the severi ty of pancreati ti s were observed・ After experiment started for five days , the activity of serum anti oxi da nt enzymes (SOD, GSH-Px) and the concentration of serum amylase in rats of each group were measured respectively. Results: The immobility time in the tail suspension test of group A and F were significantly longer than that of group C (P It;0・01). Since the second day after the experiment has started, the immobi lity time in the tail suspension test of group F was shorter than that of group A. However, only on the 4th and Sth day, this difference showed statistic significance (P It;0.05 and P lt;0・01 respectively)・ Histopathological analysis suggested the infiltration of that inflammatory cells in group F was signifleant1y weaker than that of group A (P It;0・01)・ Serum glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) level in group A were significantly lower than that in group C (P It;0.01), and these parameters of group F were significantly higher than those of group A (P It;0.01). The duration of immobility in the tai 1 susp