在癫痫发作中的作用.docxVIP

Abs仃actAbstract Abs仃act Abstract Epilepsy is a disorder of the brain characterized by an enduring predisposition to generate epileptic seizures，and by the neurobiologic，cognitive，psychological，and social consequences of this condition．There is an emerging view that the patho—mechanisms underlying the process of epileptogenesis and progression of the epileptic state，involve miRNAs．MiR-23b-3p have various biological effects that control neuron proliferation，differentiation and apoptosis and involed in a variety of pathological mechanism of neurodegenerative diseases． In this thesis，we epilepsy model by intraperitoneal injection of KA，the control group injects the same dose of saline，and the Inclusion criteria is behavioral seizure achieved Racine scale 4-5 stage．Next，we detected the expeission of miR-23b·3p，miR-505-5p and miR-55 1 b-3p in the cortex and hippocampus by fluorogenic quantitative PCR，The results reveal a significant reduction in the expression of miR一23b-3p in the cortex of KA induced mouse，moreover，the expression of miR·-23b·-3p in different dose and different time course was also declined significantly．These findings suggest that miR-23b-3p plays important roles in in both in vivo and in vitro models of epilepsy．Then，we injected miR-23b-3p agomir or control agomir into the lateral cerebral ventricle of KA model mouse．As expected，we found that seizure induced by KA in miR-23b-3p agomir co-treated Was significant alleviated，with lower amplitude and lower spike frequency．These results suggest that miR-23b-3p supplement may have anti-seizure potential． In conclusion，OUr study first discovered the expression level of miR-23b一3p is decreased in cell and animal models of epilepsy，and supply miR一23b-3p by its agomir Can alleviate seizure severity and seizure—like EEG in mice treated with KA．These result provide new target and idea for the treatment of epilepsy and a diagnostic marker for epilepsy；and established a solid foundation for further research