病毒学实验技术.pptxVIP

病毒学实验技术;viruses are usuallydetected by indirect methods;Sampling Injection in animal models (diseases), or eggs Virus culture in mosquito or mammalian cells Detection of virus replication Cytopathic effect (syncitia, inclusion bodies, cell rounding, apoptosis, microarrays) Hemadsorption Antigen detection (immunocapture, Dot-blot, Western blot, Immunofluorescence, Immunohistochemistry, ELISA Electron microscopy Genome detection (Electrophoresis, in situ or membrane Hybridization, RT-PCR, ` multiplex or real time PCR, NASBA, Hybrid RNA-DNA, microarrays, Sequencing, substraction sequencing