硕士论文trametes spsybcl3发酵产漆酶条件的优化、分离纯化、酶学性质及其应用初步研究.pdfVIP

江南大学 硕士学位论文 Trametes sp.SYBC-L3发酵产漆酶条件的优化、分离纯化、酶学 性质及其应用初步研究 姓名：刘家扬 申请学位级别：硕士 专业：生物化学与分子生物学 指导教师：廖祥儒 Abstract Abstract Laceaseisa ofmulti oxidases of a type copper-containingpolyphenolcapableoxidizing wide of andother aromatic call rangepolyphenols lignin—derived in and herbicideandwastewater and degradeligninphenoxycontaining petroleumindustry affecttoxic Canthusbe usedin phenolicmaterials；they widely paperpulp and andenvironment industry,medicationsanitation,biosensor protection． Inthis strainof laccase wasobtainedfrom thesis，a high producingmicroorganism kraurotic wasidentified observationand18S chumpby screening．It bymorphological plate as rDNA andtermedTrametes sequenceanalysis sp．SYBC—L3． Thelaccase Trametes Wasstudiedunder state productionby sp．SYBC—L3 liquid fermentation谢mdifferentmediaandfermentationconditions．The state optimizedliquid fermentationmedium 1 beanmeal contained(g／L)：maltose2，soy 6，CuS040．4，Ⅺ如P041， 0．8 0．5．The Na2HP04