Illumina 测序的原理和应用.pptxVIP

Illumina 测序的原理和应用;Illumina测序流程;Adapter and Library 接头和文库;Illumina测序流程;什么是 flow cell？;1. Single DNA libraries are hybridized to primer lawn and extended by polymerase ;2. Double-stranded molecule is denatured and original template is washed away ;3. New synthesized strand is covalently attached to flow cell surface to form a bridge and extended by polymerase 4. Double-strand bridge is formed ;5. Double-strand bridge is denatured and form two copies of covalently bound single-stranded templates 6. Bridge amplification cycle repeat ;Multiple bridges are formed dsDNA bridges are denatur