光学显微与电子成像1.pptx

Chapter 1Fundamentals of Light MicroscopyJunle QuE-mail: jlqu@深圳大学光电工程学院Microscope-显微镜 Microscope: instrument to see objects too small for the naked eye. /wiki/MicroscopeHistory of invention It is difficult to say who invented the compound microscope. It is often said that Dutch spectacle-makers Hans Janssen and his son Zacharias Janssen to invent the first compound microscope in 1600s The oldest published image known to have been made with a microscope (1630)Three Goals of MicroscopyM: produce a magnified image of the specimenR: separate the details in the imageC: render the details visible to the human eye or camera. MRC: Magnification, Resolution, ContrastHistory of Light MicroscopyA simple compound microscope invented by British microscopist Robert Hooke sometime in the 1660s. History of Light MicroscopyInvented by Robert Hooke in1600s 20th century microscopes 21th century microscopes MicroscopeMicroscope Microscopy MicroscopicMicroscope (显微镜)instrument to see objects too small for the naked eye. Microscopy (显微成像方法)the science of investigating small objects using such an instrument. Microscopic (用显微镜可见的)invisible to the eye unless aided by a microscope.Microscope ElementsBasic components of the light path：光路部分构成Ocular lens (eyepiece) (1)-目镜 Objective (3) –物镜Diaphragm and condenser lens (8) 光栏和聚光镜Light source, a light or a mirror (7) “Structural” components:结构部分 Objective turret (2) （目镜架）Stage (to hold the sample) (6 ) 、 ( 9) 样品台Focus wheel（聚焦轮） to move the stage (4 - coarse adjustment, 5 - fine adjustment) Basic optical transmission microscope elements A collection of different types of eyepiecesA 25 mm Kellner eyepieceWhat is an eyepiece (or ocular)目镜?An eyepiece, or ocular lens: is a type of lens that is attached to a variety of optical devices such as telescopes（望远镜) and microscopes(显微镜）.named because it is usually the lens that is closest to the eyeWhat is eyepiece (or ocular)? 1-Real image 2- Field diaphragm 3- Eye relief 4- Exit pupilThe eyepiece is