双酶切buffer如何选择.pdfVIP

双酶切 buffer 如何选择 Recommended Universal Buffer for Double Digestion Double digestion (cutting a DNA with two restriction enzymes simultaneously) is used widely to save time. Takara provides universal buffers and the relative activity in each reaction buffer is shown on the table Restriction enzyme activity displaying system using universal and basal buffers . In some combinations, however, it may be difficult to select suitable buffer for double digestion. In this table, the suitable buffer type, dilution rate and additive are shown to perform double digestion with enzymes that hav