文献简报2022-05-09(2022年-2023年).docxVIP

金昆 文献简报 2021-05-09 Fractal Nanoplasmonic Labels for Supermultiplex Imaging in Single Cells Jianlei Shen, Le Liang,Mingshu Xiao, Xiaodong Xie, Fei Wang,Qian Li, Zhilei Ge, Jiang Li, Jiye Shi, Lihua Wang, Li Li, Hao Pei,* and Chunhai Fan*, Journal of the American Chemical Society ( IF 14.612 ) Pub Date : 2019-07-18 DOI: 10.1021/jacs.9b03498 ABSTRACT: The rapidly increasing need for systems biology stimulates the development of supermultiplex (SM) methods for simultaneously labeling multiple biomolecules/cells with distinct colors. Here we report the development of DNA-engineered fractal nanoplasmonic labels with ultrahigh brightness and photostability for SM imaging in single cells. These color-resolvable nanoplasmonic labels have a uniform size of ～50 nm with an inner hollow gap of ～1 nm. The outer shell morphology is highly tunable with the tip aspect ratio covering the range of δ = 0.29? 1.66, which supports SM plasmonic imaging exceeding the conventional flfluorescence multiplexing limit. We demonstrate the use of these SM labels for quantitative imaging of receptor-mediated endocytosis and intracellular transport of multiple protein?NP structures in a single cell in real time. This SM-plasmonic method sheds light on elucidating complex interactions among protein?NPs in nanotoxicology and facilitates the development ofnovel nanomedicines for diagnosis and 金昆 文献简报 2021-05-09 therapy. 对系统生物学的快速增长的需求刺激了用于同时标记具有不同 颜色的多个生物分子/细胞的超级多路复用（SM）方法的发展。在这 里，我们报道了具有超高亮度和光稳定性的 DNA 工程形的分形纳米 等离子体标记的发展，用于单细胞 SM 成像。这些颜色可分辨的纳米 等离子体标记物具有约 50 nm的均匀大小和约 1 nm 的内部空心间隙。 外壳形态高度可调，尖端长宽比覆盖δ= 0.29- 1.66 的范围，支持 SM 等离子体成像超过常规的荧光复用极限。我们证明了这些 SM 标签用 于实时介导单个细胞中的受体介导的内吞作用和多种蛋白-NP 结构 的细胞内转运的定量成像。这种 SM-等离激元方法阐明了纳米毒理学 中蛋白质-NP 之间复杂的相互作用，并促进了用于诊断和治疗的新型 纳米药物的开发。 金昆 文献简报 2021-05-09 图 1 ，分形纳米等离子体标记的