HBV感染者IL18基因转录水平及其血清中含量检测的意义临床医学.doc

HBV感染者IL18基因转录水平及其血清中含量检测的意义临床医学 目录 TOC \o "1-9" \h \z \u 目录 1 正文 1 文1：HBV感染者IL18基因转录水平及其血清中含量检测的意义临床医学 1 1 对象与方法 3 2 结果 4 3 讨论 5 文2：急性脑梗死患者血清中IL 6 1 资料与方法 7 12 研究方法 8 2 结果 8 3 讨论 8 参考文摘引言： 9 原创性声明（模板） 11 文章致谢（模板） 11 正文 HBV感染者IL18基因转录水平及其血清中含量检测的意义临床医学 文1：HBV感染者IL18基因转录水平及其血清中含量检测的意义临床医学 Abstract： Objective To evaluate the possible roles of interleukin 18 (ILl8) in hepatitis B virus (HBV) infected individuals. Methods Peripheral blood lymphocytes from three groups of HHV infected individuals including 15 asymptomatic carrie, 35 patients with chronic hepatitis and 18 patients with fulminant hepatitis, and from 24 blood dono (as normal controls) were analyzed. The tracription and expression of IL18 in peripheral blood monocytes (PBMC) were assessed by semiquantitive revee tracription polymerase chain reaction (PCR) and flow cytometry. Results (1) There were significant differences in the tracription and expression levels of IL18 among the groups of infected individuals. Interestingly, it was the highest in the fulminant hepatitis group, and the lowest in the asymptomatic carrier group. However, there was no significant difference between those with chronic hepatitis and normal controls. The severity of the disease was accompanied by the elevation of IL18 production. (2) The severity of the liver inflammation in those with chronic hepatitis was companied by the elevation of IL18 production. Conclusion IL18 may take part in the host immunity agait HBV, and relate to the inflammation degree of the liver. Key words： Hepatitis B virus; interleukin18; gene expression 细胞免疫和体液免疫是清除体内乙型肝炎病毒(HBV)的主要方式，并且以细胞免疫为主。所以参与细胞免疫的各种细胞因子的作用越来越受到重视。白细胞介素18作为新近发现的细胞因子，在细胞免疫调节中起着重要的作用，它具有刺激INFγ分泌、FasL表达、增强NK细胞和Th1细胞活性等功能。近年来其在病毒性肝炎损伤过程的作用日益引起重视。本研究采用RTPCR和ELISA法测定各型乙型病毒性肝炎患者外周血单核细胞（PBMC）IL18 mRNA转录水平和血清中IL18含量，旨在探讨其在不同类型肝炎患者血清中的变化 规律 及其与肝功能受损的关系。 1 对象与方法 研究对象 为本院门诊体检及住院HBV感染者68例，女19例，男49例，年龄19～65(32±9)岁，其中无症状携带者15例，慢性肝炎35例，重型肝炎18例。 所有