深低温停循环下神经生长因子的脑保护作用研究.docVIP

深低温停循环下神经生长因子的脑保护作用研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：深低温停循环下神经生长因子的脑保护作用研究 1 1 材料和方法 2 2 结 果 4 3 讨 论 5 文2：不同灌注方法对深低温停循环下脑保护的实验研究 6 1 材料与方法 8 2 结 果 10 3 讨 论 10 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 深低温停循环下神经生长因子的脑保护作用研究 文1：深低温停循环下神经生长因子的脑保护作用研究 Abstract: OBJECTIVE To establish a model of deep hypothermie circulatory arrest(DHCA) and study the neural apoptosis in the hippocampus and cerebral cortex, investigate the protective effects of nerve growth factor(NGF) during DHCA． METHODS Twenty healthy baby rabbit()were randomly divided into two groups(10 rabbits per group): study group (preconditioning with NGF) and control group (without NGF). S100β content of blood taken in different window around DHCA was measured．Neural apoptosis was identified by TUNEL in hippocampus CA1 region and cerebral cortex of brain．Comparely analysis the change of S100β and the expression of neural apoptosis in the hippocampus and cerebral cortex between two group. RESULTS The number of neural apoptotic cells in study group were decreased significantly during DHCA (P). Serum S100β levels were significantly difference at post DHCA 30min(T2),1 h(T3),3 h(T4),5h(T5),12h(T6) between two group(P). CONCLUSION Exogenous NGF inhabit Neural apoptosis of hippocampus and cerebral cortex and has a protective effect on neuronal injury induced by DHCA. Key words： Deep hypothermie circulatory arrest; Nerve growth factor 深低温停循环(deep hypothermic circulatory arrest，DHCA)能为心脏及大动脉手术提供一个无血最佳手术视野，但停循环可使全身重要器官尤其是大脑处于缺血、缺氧状态，造成一定程度上的脑组织损伤,如何预防这种损伤是近年来的研究热点。近期许多研究证明神经生长因子(nerve growth factor，NGF)可通过多种机制减少缺血／再灌注神经元的凋亡［1-3］，同时已有研究证明S100β蛋白可作为体外循环(extracorporeal circulation,ECC)心脏手术后评价脑损伤的可信指标［4］。因此本实验拟采用颈总动脉与锁骨下动脉阻断的DHCA模型，以S100β蛋白为标准观察NGF对DHCA下脑组织神经细胞凋亡等损伤的影响，探讨DHCA下的脑保护方法，为后期研究提供理论依据。 1 材料和方法 材料与分组 实验动物选用健康新西兰大白兔幼兔(上海松江实验动物中心)20只，雌雄各半，体重 kg，随机分为2组，每组10只。实验组：DHCA中给予NGF；对照组：DHCA中不给予NGF。 麻醉与监护 每只动物在实验前禁食6 h，速眠新( ml/kg)诱导后气管插管，呼吸机(Newpont l00)辅助呼吸，呼吸道内正压通气。给予肌松剂维库溴铵( mg/kg，静脉注射)及