α双炔失碳酯对激素依赖人前列腺癌细胞LNCaP生长的影响临床医学.docVIP

α双炔失碳酯对激素依赖人前列腺癌细胞LNCaP生长的影响临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：α双炔失碳酯对激素依赖人前列腺癌细胞LNCaP生长的影响临床医学 1 1 材料和方法 3 2 结果 6 3 讨论 7 文2：TNP┐470对结肠癌Lovo细胞生长的抑制作用医学 8 图1略 12 图2略 12 图3略 13 参考文摘引言： 13 原创性声明（模板） 15 文章致谢（模板） 15 正文 α双炔失碳酯对激素依赖人前列腺癌细胞LNCaP生长的影响临床医学 文1：α双炔失碳酯对激素依赖人前列腺癌细胞LNCaP生长的影响临床医学 ABSTRACT: Objective To investigate the effects of αanordrin（αANO）on the growth of androgendependent human prostate cancer cell line LNCaP. Methods The monome of αANO and βANO were purified through alumina column chromatographic separation. SRB staining method was used to detect the effects of αANO on the growth of LNCaP cells and on antagonizing R1881(a kind of androgen stimulant) in inducing LNCaP cells proliferation. Cell morphology was examined under microscope. ELISA was used to examine the effect of αANO on PSA secretion by LNCaP cells. Results In normal medium，after LNCaP cells were treated with αANO at 8,12,16,24 and 32 μmol/L，cell survival rates were %, %, %, %, % respectively. In the androgendepleted medium, αANO could antagonize LNCaP cell proliferation induced by R1881. The PSA secretion of LNCaP cells was also decreased. Conclusion αANO inhibits the proliferation of androgendependent human prostate cancer cell line LNCaP. In the presence of androgen, αANO antagonizes the LNCaP cell proliferation induced by androgen. KEY WORDS: prostatic neoplasms; noranotrostanes; selective estrogen receptor modulato; prostate specific antigen; cell line, tumor 选择性雌激素受体调节剂（selective estrogen receptor modulato,SERM）是指能与雌激素受体相互作用，依据靶组织和激素内环境的不同而产生特异作用的-类化合物。它们对雌激素有双相作用，即能抗雌激素又具有拟雌激素样作用。SERM已被证实可 治疗 乳癌、骨质疏松和心血管疾病；并可能预防前列腺癌（prostate cancer, Pca）[12]。寻找新的理想的SERM是目前研究热点。 双炔失碳酯（2α，17α双乙炔基A环失碳雄烷2β，17β双羟基双丙酸酯,anordrin,ANO）是甾体A环失碳雄甾烷衍生物[3]，临床上用于抗着床、抗早孕。药理活性研究表明ANO具有强的抗雌激素作用和弱的雌激素样活性[4]，与SERM类药物特点相似。 文献 报道，ANO在合成过程中，同时生成2个炔基构型不同的异构体: αANO和βANO。αANO对多种肿瘤细胞包括HL60、人卵巢癌细胞等有直接杀伤作用[5]。笔者观察αANO对激素依赖人前列腺癌细胞LNCaP生长的影响，报道如下。 1 材料和方法 材料 试剂与仪器 ANO