吡咯烷二硫氨基甲酸酯对犬心肌线粒体损伤的保护作用.doc

吡咯烷二硫氨基甲酸酯对犬心肌线粒体损伤的保护作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：吡咯烷二硫氨基甲酸酯对犬心肌线粒体损伤的保护作用 1 1 资料与方法 3 2 结 果 4 3 讨 论 5 文2：NF－κB抑制剂吡咯烷二硫氨基甲酸对人翼状胬肉成纤维细胞增殖的影响 6 1材料和方法 8 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 14 正文 吡咯烷二硫氨基甲酸酯对犬心肌线粒体损伤的保护作用 文1：吡咯烷二硫氨基甲酸酯对犬心肌线粒体损伤的保护作用 Abstract: OBJECTIVE To observe the effect of pyrrolidine dithiocarbamate(PDTC)on canine myocardial mitochondrial injury during extracorporeal circulation (ECC). METHODS Twelve healthy mongrel dogs undergoing ECC were randomly allocated into control group(group C,n=6)and PDTC pretreatment group(group P,n=6).All dogs were anesthesitized with % sodium pentobarbitone 25 mg/ tracheal intubation,the myocardial ischemia-reperfusion injury model during ECC was made by declamping(DC)after aortic cross-clamping(AC)for 60 group P,PDTC 20 mg/kg was administered intravenously before ECC，while in group C physiological saline was given itead of mitochondria were isolated before ECC,60 min after AC，30 min and 60 min after DC for determination of superoxide dismutase (SOD),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),total anti-oxidation capacity (T-AOC) and mitochondrial swelling degree(MSD). RESULTS The myocardial mitochondria of SOD,GSH-PX and T-AOC were decreased，while MDA and MSD were increased after AC as compared to the value before ECC in both groups(P＜).SOD,GSH-PX and T-AOC were decreased，while MDA and MSD were increased after DC as compared to the value before ECC in group C(P＜).The myocardial mitochondria of group P at 60 min after DC had not significant differences in content of MDA,SOD,GSH-PX,T-AOC and MSD(P＞).SOD,GSH-PX and T-AOC were distinctly lower，while MDA and MSD were distinctly higher at 60 min after AC and after DC in group P than those in group C(P＜). CONCLUSION Pretreatment with PDTC is effective in improving the anti-oxidation ability of myocardial mitochondria and attenuate myocardial mitochondrial injury. Key words： Pyrrolidine dithiocarbamate;Mitochondria;Extracorpo