皮胶原黄变机理及抑制黄变方法的的研究.pptVIP

皮胶原黄变机理及抑制黄变方法的研究Yellowing mechanism and methods of avoiding yellowing of skin collagen 指导老师 ：汤克勇 教授 汇 报 人 ：李双雯 1 Background and Purpose 2 Research content 3 Experiment 4 Later Scheme Background and Purpose clothes bag sofa shose skin care product foodstuff health food gelatin albumen powder Synthetic Polymer Materials Yellowing cellulose lignin fibroin Natural Polymer Skin material properties primary structure conformation microstructure The relationship between collagen structure and sensitivity to UV radiation Research content Yellowing of skin collagen Light screening agent Yellowing mechanism The methods of avoiding yellowing film solution ZnO TiO2 SiO2 pretreatment Extraction Centrifugation salting-out …… dialysis hydrolysis for 72h mechanical smash 0.5 mol/L CH3COOH 4 oC ,96 h Extraction of collagen Experiment Fig1. SDSof collagen Ι extracted from porcine skin by pepsin Fig 2. The relationships between lg Mr and Relative Mobility of Protein Molecular Weight Marker collagen Ι Mr ≈ 392.5 KDa Solution of Collagen ? c=3.335 mg/g Solution of Collagen ? PH≈ 6 Light stabilizer UV-312 nm Collagen Films Fig.4 The whiteness of collagen films irradiated for different time Fig.3 UV-vis transmittance spectra of collagen films irradiated for different time Fig.5 FTIR difference spectra of collagen film and collagen film exposed on UV for 32h Amide A Amide B Amide ?? Amide ? Collagen solution Fig 6. Photos of collagen after freeze drying (a)UV-0h(b)UV-32h(c)UV-2h a b c Fig 7. SEM images of collagen fiber(a)UV-0h; (b)UV-32h;(c)UV-2h a b c Fig. 8 A plot of relative viscosity (η/η0) against time