17、分子生物学课件4个mb3.pptxVIP

Review of last lecture (1);Genome complexity C value paradox Genome complexity Reassociation rate (Cot1/2);Methods of Molecular Biology(Chapter 4 and 5);Molecular cloning methods; 1000bp GOI DNA 3 x109 genomic DNA;Principle of gene cloning;In vitro gene cloning (PCR);PCR process;Principle of PCR;Cycles of PCR reactions There are 3 major steps in a PCR, which are repeated for 30 or 40 cycles. This is done on an automated cycler, which can heat and cool the tubes with the reaction mixture in a very short time. Denaturation at 940C: double strand opens to single stranded DNA Anneal