重庆地区幽门螺杆菌对甲硝唑的耐药性和耐药机制的研究.docVIP

重庆地区幽门螺杆菌对甲硝唑的耐药性和耐药机制的研究 文档信息 属性： Doc-03VRSV，doc格式，正文10412字。质优实惠，欢迎下载！ 说明： 作为医药学资料、药学资料的写作参考资料，提供解决怎么写及格式等相关问题。 适用： 作为文章写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：重庆地区幽门螺杆菌对甲硝唑的耐药性和耐药机制的研究 2 1 材料和方法 3 2 结果 5 3 讨论 5 文2：重庆地区幽门螺杆菌对甲硝唑的耐药性和耐药机制的研究药学论文 7 1 材料和方法 8 2 结果 10 3 讨论 11 参考文摘引言： 12 原创性声明（模板） 14 文章致谢（模板） 14 正文 重庆地区幽门螺杆菌对甲硝唑的耐药性和耐药机制的研究 文1：重庆地区幽门螺杆菌对甲硝唑的耐药性和耐药机制的研究 ABSTRACT Objective To investigate the prevalence and resistance mechanism of Helicobacter pylori(Hp) resistant to metronidazole in Chongqing area，and itruct clinical medication. Methods Ninety six Hp clinical isolates were cultured. The susceptibilities of the strai to metronidazole were tested by agar dilution test. Then compared the prevalence of Hp resistant to metronidazole in urban and in rural area. Of 44 Hp resistant strai to metronidazole were selected. The DNAs of Hp were extracted and prepared for PCR to amplify rdxA gene. The PCR amplification products were sequenced after TA cloning. Then， the sequences were compared with NCTC11637. Results The prevalence of Hp resistant to metronidazole in Chongqing area was %. There was no statistically significant difference between the urban area and the rural area (P). In comparison with NCTC11637， mutatio caused by base iertion and substitution in rdxA of the resistance isolates were found. Conclusio The results indicated that the prevalence of Hp resistant to metronidazole in Chongqing area was higher. In this aspect， metronidazole was used to treat Hp no longer. Additionally， the majority of metronidazoleresistant Hp strai in Chongqing have gene mutation in rdxA， it may be the key factor that causes metronidazoleresistant Hp in Chongqing。 KEY WORDS Heliobacter pylori; Metronidazole; Resistance; Mutation 幽门螺杆菌(Heliobacter pylori，Hp)是一种革兰阴性微需氧菌，自Mahall和Warren首先用微需氧技术分离出Hp之后，人们相继发现Hp与慢性胃炎、消化性溃疡、胃癌、胃黏膜相关淋巴组织淋巴瘤、心血管疾病、自身免疫性疾病、皮肤病等有密切关系［1，2］。全球约一半的人口感染过Hp，通常在儿童时就已感染，若