生物质谱：质谱常见扫描方式.ppt

质谱常见扫描方式 四极杆 1、Full Scan MS全扫描功能 几种常见扫描模式 Precursor (Parent) ion scan Product ion scan Neutral Loss Scan Full scan （全扫描） Selected ion monitoring （选择离子监控） Selected reaction monitoring （选择反应监控） Multiple reaction monitoring （多级反应监控） Multiple reaction monitoring To detect the selected fragment from the target analyte for identification or quantification analysis MRM 扫描方式的特点 多组分同时分析 共流出物的分析 母离子-子离子关系明确， 定性能力强 复杂样品、脏样品的分析 同位素内标的使用 可区分同分异构体 Company Logo L o g o * L o g o single mass transmission mode mass scanning mode m1 m3 m4 m2 m3 m1 m4 m2 m2 m2 m2 m2 m3 m1 m4 m2 + Quadrupole Triple Quadrupole Mass Spectrometer CAD Gas N 2 Declustering Quadrupole Quadrupole 1 Collision Quadrupole 3 + + + + + + + + + + Detector + + + + + + Single Quadrupole Mass Spectrometer c N 2 + Declustering Quadrupole Quadrupole 1 + + + + + Fragmentation + Orifice CID CID MS MS MS Precursor Scan Neutral Loss SIM SRM Full scan Product ion scan MRM Q1 Q2 Q3 m/z= 30 (Fixed) m/z= 50 - 200 (Scanning) CAD gas on C.E.= 20eV + + + + + + Neutral species To look for the precursor of the fixed fragment ion To search for the derivatives e.g. metabolite Precursor Scan MS/MS Q3 Q2 Q1 MS Mode = scanned; = selected m/z; = CID 适合于含有相同局部结构的不同化合物的鉴定 m/z= 50 - 300 (Scanning) m/z= 250 (Fixed) CAD gas on C.E.= 20eV + + + + + To identity the fragmentation pattern of certain molecule Product ion scan Q3 Q2 Q1 MS Mode = scanned; = selected m/z; = CID 适合于化合物官能团分析以及化合物的结构解析 m/z= 50 - 350 (Scanning) m/z= 100 - 400 (Scanning) CAD gas on C.E.= 20eV Loss of 50 Da + + + + + + Neutral species To identity a group of chemical that loss a neutral fragment Loss of CO2 in -COOH containing chemicals Neutral Loss Scan Q3 Q2 Q1 MS Mode = scanned; = scanned with offset; = CID 适合于含某一官能团的化合物鉴定 Full scan (Q3) Full scan (Q1) Q3 Q2 Q1 MS Mode = scanned; = selected m/z; blank = complete transmission 可获得丰富的谱图信息 适合未知化合物的结构鉴定 Selected ion monitoring (SIM) (Q3) Selected ion monitoring (SIM) (Q1) Q3 Q2 Q1 MS Mode = scanned; = selected m/