HPLC法同时测定血府逐瘀水蜜丸中3个易热解成分的含量.docVIP

HPLC法同时测定血府逐瘀水蜜丸中3个易热解成分的含量 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：HPLC法同时测定血府逐瘀水蜜丸中3个易热解成分的含量论文 1 1仪器与材料 3 2方法与结果 4 22溶液制备 4 24方法学考察 5 文2：HPLC法同时测定壮骨关节丸中3种有效成分的含量 7 1 仪器与试药 8 2 方法与结果 9 3 讨 论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 HPLC法同时测定血府逐瘀水蜜丸中3个易热解成分的含量 文1：HPLC法同时测定血府逐瘀水蜜丸中3个易热解成分的含量论文 Simultaneous Determination of Three Labile Pyrolysis Components in Xuefuzhuyu Water-honeyed Pill by HPLC Shangguan Tongqiang1HAN Fengyu3YU Xiuling1*QI Ping2LI Hongze2 Mongolia Tianqi HanMongolia Pharmaceutical Co， Chifeng 024000，China； MongoliaInner Mongolia Boao Modern Mongolian-Chinese Medicine Technology Research co， Chifeng 024000，China； Mongolia TianqiPharmaceutical industry investment Co， Chifeng 024000，China Abstract：Objective To establish an HPLC method for the dete1rmination of catalpol、hydroxysafflor yellow A、ligustilide in Xuefu Zhuyu Water-honeyed PillMethods Three target components were separated byDIKMA Diamoil C18（2） column （250 mm × 46 mm， 50 μm） with a gradient mobile phase coisting of methanol-acetonitrile （80 ∶[KG-*2]20， A）-05% phosphoric acid （B） （0～80 min， 98% B； 80～200 min， 98% - 75% B； 200～210 min，75% - 40% B；210～400 min，40% B）at a flow rate of 10 mL/min，and simultaneously quantificated by variable detection wavelength： 0～80 min， 210 nm； 80～250 min， 403 nm； 250～400 min， 280 nmThe column temperature was 30℃and injection volume was 10 μLResults The calibration curve of catalpol、hydroxysafflor yellow A and ligustilide were linear within 0199～1989μg（r2=09995）， 00392～0392μg（r2=09991） and 00204～0204μg （r2=09992）The average recovery rates were 9921%～10092%， D was all below 20%The average contents of catalpol、hydroxysafflor yellow A and ligustilide in Xuefu Zhuyu Water-honeyed Pill were 007%， 009% and 0051%Conclusion The method is simple， accurate and reliable for the quality control of Xuefu Zhuyu Water-honeyed Pill Keywords：Xuefu Zhuyu Water-honeyed Pill； HPLC； Catalpol ；Hydroxysafflor yellow A； Ligustilide 血府逐瘀丸由血府逐瘀汤剂改成，