心脏电生理技术基础(膜片钳).ppt

CELLULAR CARDIAC ELECTROPHYSIOLOGICAL TECHNIQUES;Electrical model of the membrane Standard intracellular microelectrode technique Voltage clamp technique Patch clamp technique;G=1/R;Ohm’s law;Current clamp;Intracellular microelectrode technique;编辑课件;0.1 - 0.2 ?m;编辑课件;编辑课件;The setup;;;The voltage-clamp circuit;Patch-clamp: the special case of the voltage clamp ;;;;closed open;closed open open;;;;;;;;;;;;;;编辑课件;;NaCl 144 NaH2PO4 0.4 KCl 4 MgSO4 0.53 CaCl2 1.8 Glucose 5.5 HEPES 5 + ICa blocker;;编辑课件;The “run-down“ effect;The L-type calcium current;;The “run-