循环的microRNA是早期诊断人类急性心肌梗死的潜在的新型生物标志物.ppt

Circulating microRNA: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans 第一页，共十六页。 MicroRNA (miRNA) is reported to be present in the blood of humans and has been increasingly suggested as a biomarker for diseases. We aim to determine the potential of cardiac-speci?c miRNAs in circulation to serve as biomarkers for acute myocardial infarction (AMI). Aims 第二页，共十六页。 (1)By verifying their tissue expression patterns with real-time polymerase chain reaction (PCR) analysis, muscle-enriched miRNAs (miR-1, miR-133a, and miR-499) and cardiac-specific miR-208a were selected as candidates for this study. Methods and results (2)With miRNA microarray and real-time PCR analyses, miR-1, miR-133a, and miR-499 were present with very low abundance, and miR-208a was absent in the plasma from healthy people. In the AMI rats, the plasma levels of these miRNAs were significantly increased. Especially, miR-208a in plasma was undetected at 0 h, but was significantly increased to a detectable level as early as 1 h after coronary artery occlusion. 第三页，共十六页。 Methods and results (3)By receiver operating characteristic(ROC) curve analysis, among the four miRNAs investigated, miR-208a revealed the higher sensitivity and specificity for diagnosing AMI. Further evaluation of the miRNA levels in plasma from AMI patients (n =33) demonstrated that all four miRNA levels were substantially higher than those from healthy people (n =30, P , 0.01), patients with non-AMI coronary heart disease (n =16, P , 0.01), or patients with other cardiovascular diseases (n =17, P , 0.01). Notably, miR-208a remained undetectable in non-AMI patients, but was easily detected in 90.9% AMI patients and in 100% AMI patients within 4 h of the onset of symptoms. 第四页，共十六页。 Results Figure 1 Tissue expression patterns of microRNAs in rat and human tissues. (A) Real-time polymerase chain reaction analysis of microRNA expression in various tissues from rat. (B) Real-