Agilent MassHunter Workstation Software - Data Acquisition for 6410 Triple Quad Familiarization Guide说明书用户手册.PDF

Agilent MassHunter Workstation Software – Data Acquisition for 6410 Triple Quad LC/MS Familiarization Guide Before you begin 3 Prepare your system 3 Prepare to acquire data 3 Exercise – Develop an acquisition method for the 6410 5 Task 1. Enter acquisition parameters and acquire data 5 Task 2. Determine precursor ion masses 10 Task 3. Find optimum fragmentor voltage for maximum response 13 Task 4. Determine product ion masses 23 Task 5. Find optimum collision energy for MRM acquisition 30 Use the exercise in this guide to learn to use the Agilent 6410 Triple Quad LC/MS. You can do this exercise with the demo data files, SulfaDrugs, shipped with the system (in the Data folder of your Data Acquisition instal- lation disk), or with data you acquire. With this exercise, you learn how to determine the best acquisition settings for analyzing your compounds of interest. These instructions help you understand not only how to set up a worklist to optimize instrument parameters for best sensitivity in acquisition, but also how to use the Qualitative Analysis program to identify parameter values producing optimum signal response. You can also learn about the Qualitative Analysis program by using the Qualitative Analysis Familiarization Guide. Agilent Technologies In this exercise, you create an acquisition method for a mixture of four sulfa drugs, optimizing both the fragmentor and collision energy voltages to maximize sensitivity. One of the ways to optimize parameters is to create a worklist, or sequence, of data file acquisitions, each using a different method. This exercise uses this protocol for method development. Another way to develop a method is to use the manual tune capability to optimize