Agilent Primary Characterization of a Monoclonal Antibody Using Agilent HPLC-Chip Accurate-Mass LC MS Technology说明书用户手册.PDF

Application Note Primary Characterization of a Monoclonal Antibody Using Agilent HPLC-Chip Accurate-Mass LC/MS Technology The characterization of a monoclonal antibody at low nanogram levels using Authors an Agilent HPLC-Chip system coupled to an Agilent Accurate-Mass Q-TOF MS instrument is described. The superior sensitivity and mass accuracy of the Ravindra Gudihal HPLC-Chip LC/MS platform, combined with the powerful data processing Sudha Ragalopalan Ranjit Gulge capabilities of Agilent MassHunter and BioConfi rm software, enabled easy and Andrew Gieschen rapid identifi cation of antibody heterogeneity and cleaved fragments. Robust, Christine Miller reliable, and easy to use, Agilent nanofl ow LC/MS technology is ideally suited Ning Tang for the routine analysis of biopharmaceuticals. Keith Waddell Agilent Technologies, Inc., Introduction Santa Clara, CA, USA Recombinant monoclonal antibodies (mAbs) represent an important class of bio- pharmaceutical products with a wide range of diagnostic and clinical applications. The worldwide market for monoclonal antibodies is projected to reach $26 billion by