Agilent Technologies Optimizing Fraction Collection with LC MSD Systems Using an Active Splitter and Delay Sensor 技术手册说明书.PDF

Optimizing Fraction Collection with LC/MSD Systems Using an Active Splitter and Delay Sensor Technical Note Wayne Duncan and Douglas McIntyre Agilent Technologies Introduction The extensive and growing research in genomics delay sensor are an integral part of the Agilent and proteomics has lead to many more targets for purification/analysis platform and provide users small-molecule drug candidates. There is also a with powerful purification flexibility while maxi- corresponding growth in the number and quality mizing efficiency and ease of use. of drug candidates required for screening. Reli- able and meaningful biological testing depends to The data shown in this note were generated using a large extent on obtaining drug candidates with an Agilent 1100 Series LC/MSD SL quadrupole minimal amounts of impurities and side-products. mass spectrometer system (G2708DA), G1361 This has created a strong need for robust and effi- preparative pumps, G2260A preparative autosam- cient high-throughput systems for compound iden- pler, G1315A diode array detector (DAD), and tification, purification, and quantification. With G1364A fraction collector. In addition to the the growing trend to higher-purity compounds, ChemStation software (G2710 Rev. A.09.01) synthetic chemists typically want to concentrate the system included Purify software (G2262 , on organic synthesis issues and minimize the time G2263 , and G2264 Rev. A.01.00) and Easy spent on the analysis and purification of their Access for Purify software (G2726 Rev. A.01.00). synthesis products. The Agilent active spl