Agilent Achieving the best recovery results with the Agilent 1100 Series dual-loop autosampler PS Quick Reference Guide说明书用户手册.PDF

Achieving the best recovery results with the Agilent 1100 Series dual-loop autosampler PS Quick Reference Guide CAUTION Important Information! Please read before using the instrument! Introduction The injection principle of the dual-loop autosampler PS is different to that of other Agilent 1100 Series autosamplers. It is a fixed-loop push-through design as shown in Figure 1. Since the sample has to be drawn into a buffer loop and then transferred into the injection loop several items have to be considered to achieve best recovery results. Figure 1 Injection principle dual-loop autosampler Agilent Technologies Rinse solvent • The rinse solvent is used to rinse the injection port, the seat capillary and the injection valve after sample injection. • To avoid precipitation problems, use a solvent in which the sample is readily soluble. For reversed-phase chromatography this could be acetonitrile, methanol or DMSO/acetonitrile 50:50 v/v, for example (pure DMSO is not recommended due to its high viscosity, see Sample draw and eject speed). • We strongly recommend to use the Rinse function subsequently to each injection. The rinse volume, which is calculated from the injection volume and a user-defined factor, should be at least 300 µL. • The syringe should be purged at least 5 times using th