Agilent The Separation of Seven Synthetic Artificial Food Colors on Agilent HC(2) TC(2) Reversed Phase Columns 说明书用户手册.PDF

The Separation of Seven Synthetic/Artificial Food Colors on Agilent HC(2)/TC(2) Reversed Phase Columns Application Food and Flavors Author possible harmful effects of these compounds, including allergic reactions and hyperactivity in Rongjie Fu children. Artificial food colorings are restricted in Agilent Technologies, Inc. some countries because of these possible effects. 412 Ying Lun Road The quantity of these compounds in food quality Pu Dong, Shanghai 200131 control is becoming more important, as is the need China to prove that they meet international food quality control standards. The structures of seven col- orants, mostly azo dyes, are shown in Figure 1. Abstract These compounds are used in beverages to give them a colorful, attractive appearance. Many synthetic or artificial colorants (Red #33, Sunset yellow, etc.) are used in food and beverages to improve In this application, we focused on developing a product appearance. These compounds can be easily sepa- method for separating seven colorants on the Agi- rated by reversed-phase liquid chromatography. [1] A new lent TC-C18(2) column and then applying this C18 column was us