Agilent Determination of the Dynamic Range for the 1200L Electrospray (ESI)-LC MS MS Using a \ CNS\ Drug and its Major Hydroxylated Metabolite as the Test Compounds Application Note说明书用户手册.PDF

Application LC/MS Note Number 4 April 2003 Determination of the Dynamic Range for the Varian 1200L Electrospray (ESI)-LC/MS/MS Using a “CNS” Drug and its Major Hydroxylated Metabolite as the Test Compounds R. Morrow and D. Garteiz, TexMS Analytical Services, Houston, TX Introduction Experimental Procedure The objective of this work is to establish the dynamic range of The Varian 1200L was used in its Selected Reaction Monitoring the Varian 1200L using TX1360 and its major OH-Metabolite (SRM) mode. TX1360, TX1370 and Buspirone calibration as the model compounds. The internal standard selected for solutions covering a range from 50 fg/µL to 80 ng/µL were this work, Buspirone, is a structurally related central nervous accurately prepared by serial dilution. A fixed amount of the system (CNS) drug. internal standard at 50 pg/mL was added to all calibration points. An “on-column” injection of 10 µL for each calibration Background standard was used. TX1360 is a pharmaceutical agent currently under development as a CNS drug. A large number of Absorption Distribution, Metabolism, and Excretion (ADME) studies as well as Pharmacokinetic (PK) studies have been completed during HPLC Conditions the development of TX1360.