Characterization of recombinant monoclonal IgG2 antibodies using LC-MS and limited 英文文献原版.docx
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Accepted Manuscript
Characterization of recombinant monoclonal IgG2 antibodies using LC-MS and limited Lys-C digestion
Christine Nowak, Rekha Patel, Hongcheng Liu
PII: S1570-0232(18)30547-6
DOI: doi:10.1016/j.jchromb.2018.05.010
Reference: CHROMB 21174
To appear in:
Received date: 3 April 2018
Revised date: 8 May 2018
Accepted date: 10 May 2018
Please cite this article as: Christine Nowak, Rekha Patel, Hongcheng Liu , Characterization of recombinant monoclonal IgG2 antibodies using LC-MS and limited Lys-C digestion. The address for the corresponding author was captured as affiliation for all authors. Please check if appropriate. Chromb(2017), doi:10.1016/ j.jchromb.2018.05.010
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT
Characterization of recombinant monoclonal IgG2 antibodies using LC-MS and limited
Lys-C digestion
Christine Nowak, Rekha Patel and Hongcheng Liu*
Product characterization, Alexion Pharmaceuticals, 100 College Street, New Haven, CT06510
* Corresponding author
Email: LiuH@
Telephone: 203-271-8354 ACCEPTED MANUSCRIPT
ACCEPTED MANUSCRIPT
ACCEPTED MANUSCRIPT
Abstract
Recombinant monoclonal antibodies have been routinely characterized at intact, subunit
and peptide levels by LC-MS. Papain and pepsin have been the enzymes commonly used to
cleave IgG into various fragments to facilitate in-depth characterization. However, non- specific
cleavage for both papain and pepsin and the need of a reducing reagent for papain has limited
their usage. In contrast, IdeS has gained popular
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