课件 文献 资料Methods for assessing the statistical si叩ficance of molecular sequence features by using general scoring schemes.pdfVIP

Proc. N叫. Arnd. Sci. USA Vol. 87会 pp. 2264-2268. March 1990 Evolution Methods for assessing the statistical si叩ficance of molecular sequence features by using general scoring schemes （艾quence alignment/prot吐n 沈quence features) SAMUEL KARLlN t AND STEPHEN F. AL TSCHUL土§ Depanment of Ma如matics噜 Stanford University. Stanford韦 CA 94305: and 之National Center for Biotechnology Information. National Library of Medicine, Natio邸l Institutes of Health、 Bethesda. MD 20894 Contributed by Samuel Karlin. December 26, 1989 ABSTRACT An unusual pattern in a nucleic acid or pro­ pendencies. For these models, theoretical results (distribu­ tein sequence or a region of strong similarity sh江ed by two or tional properties) have previously been obtained for a variety more sequences may have biological significance. It is therefore of sequence statistics such as the length of the longest run of desirable to know whether such a pattern can have arisen a given letter or pattern (allowing for a fixed number of simply by chance. To identify interesting哟uence patterns, errors), the length of the longest word (oligonudeotide, appropriate scoring val氐s can be assigned to the individual peptide) in a sequence satisfying a prescribed relationship residues of a single sequence or to sets of r函dues when several (e.g., r-fold repeat, dyad pairing), and counts and s严cings of 沈quences are compared. For single seque贰氐such scores can long repeats (5-14). Several of these analyses have been re如biophysical properties such as charge, volume, hydro­ extended to deal with comparisons within and between phobicity, or secondary structure potential; for multip